P-99: Evaluation of Pluripotency Markers of Mouse Endometrial Tissue in Different Stages of Estrous Cycle

Background: It is assumed that adult stem/progenitor cells are responsible for cycling remodeling of the uterus endometrium throughout the reproductive life of the female. This study aimed to identify and localize stem/progenitor cells in the mice uterus using immunohistochemistry. Materials and Methods: 6-8 weeks old virgin female NMRI mice were submitted to the vaginal smear examination to determine the estrous stages and those which were in estrous and di-estrous stage were selected. Immunohistochemical staining of pluripotent markers on middle region of uterus horn was performed to evaluate the association between expression of NANOG and OCT4 markers in the endometrial tissue and estrous stages. Results: Results showed that NANOG AND OCT4 markers were detectable in the both estrous and di-estrous phase in the endometrial tissue, while in the estrous phase showed a greater extent. Conclusion: Mice endometrial tissue contains stem cells and appears their status is coordinate with the hormonal changes associated with estrus phases.